Analytical Biotechnology. Capillary Electrophoresis and by Csaba Horvath, John G. Nikelly

By Csaba Horvath, John G. Nikelly

content material: High-resolution nanotechnique for separation, characterization, and quantitation of micro- and macromolecules : capillary electrophoresis / Noberto A. Guzman, Luis Hernandez, and Shigeru Terabe --
functions of capillary quarter electrophoresis to quality controls / R.G. Nielsen and E.C. Rickard --
research of cyclic nucleotides via capillary electrophoresis utilizing ultraviolet detection / Luis Hernandez, Bartley G. Hoebel, and Noberto A. Guzman --
On-column radioisotope detection for capillary electrophoresis / Stephen L. Pentoney, Jr., Richard N. Zare, and Jeff F. Quint --
recommendations for an analytical exam of organic prescribed drugs / Eleanor Canova-Davis, Glen M. Teshima, T. Jeremey Kessler, Paul-Jane Lee, Andrew W. Guzzetta, and William S. Hancock --
Analytical chemistry of healing proteins / R.M. Riggin and N.A. Farid --
development and experimental validation of protein impurity immunoassays for recombinant DNA items / Vincent Anicetti --
minimal variance purity keep watch over of preparative chromatography with simultaneous optimization of yield : an online species-specific detector / Douglas D. Frey --
Amino acid sequence-mass spectrometric analyses of mating pheromones of the ciliate Euplotes raikovi / R.A. Bradshaw, S. Raffioni, P. Luporini, B. Chait, T. Lee, and J. Shively --
Micropellicular sorbents for quick reversed-phase chromatography of proteins and peptides / Krishna Kalghatgi and Csaba Horváth --
Displacement chromatographic separations on [beta]-cyclodextrin-silica columns / Gyula Vigh, Gilberto Quintero, and Gyula Farkas.

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100 cm length) in open-tubular operation, we have collected purified proteins and peptides in nanograms quantities (85), which could be a sufficient peptide sample for microsequencing analysis. In addition, with capillary bundles, a device can be constructed that is capable of holding multiple detectors (one for every capillary column) and consequently capable of performing several simultaneous electrophoretic separations. , acrylamide) yielding a f approximatel 1 f analyt (9,22). Analytical or micro-preparative operation in capillary electrophoresis does not appear to alter the integrity of the holomeric structure of proteins, and consequently all their biological activities maintained.

21. Rose, D. ; Jorgenson, J. W. Anal. Chem. 1988, 60, 642-648. 22. Rose, D. ; Jorgenson, J. W. J. Chromatogr. 1988, 447, 117-131. 23. ; Dovichi, N. J. Science 1988, 242, 562-564. 24. Pentoney, S. ; Burgi, D. ; Zare, R. N. Anal. Chem. 1988, 60, 2625-2629. 25. Christensen, P. ; Yeung, E. S. Anal. Chem. 1989, 61, 1344-1347. ; ACS Symposium Series; American Chemical Society: Washington, DC, 1990. Chapter 3 Analysis of Cyclic Nucleotides by Capillary Electrophoresis Using Ultraviolet Detection 1 2 3,4 Luis Hernandez , Hartley G.

1987, 70, 610-617. 2. Riggin, R. ; Dorulla, G. ; Miner, D. J. Anal. Biochem. 1987, 167, 199-209. 3. Riggin, R. ; Shaar, C. ; Dorulla, G. ; LeFeber, D. ; Miner, D. J. J. Chromatogr. 1988, 435, 307-318. 4. Sittampalam, G. ; Ellis, R. ; Miner, D. ; Rickard, E. ; Clodfelter, D. K. J. Assoc. Off. Anal. Chem. 1988, 71, 833-838. 5. Becker, G. ; Bowsher, R. ; MacKellar, W. ; Poor, M. ; Tackitt, P. ; Riggin, R. M. Biotech. Appl. Biochem. 1987, 9, 478-487. ; ACS Symposium Series; American Chemical Society: Washington, DC, 1990.

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