Advances in Research and Applications by Robert S. Harris, Paul L. Munson, Egon Diczfalusy, John

By Robert S. Harris, Paul L. Munson, Egon Diczfalusy, John Glover, Kenneth V. Thimann, Ira G. Wool and John A. Loraine (Eds.)

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08 nmole/ml) ; ( * . 11 nmole/ml). 4. For excitation spectra emission a t 523 nm and an excitation slit of 8 nm, for emission spectra excitation a t 445 nm and emission slit 8 nm were used. From Kearney e l al. (1971b). 26 THOMAS P. SINGER A N D WILLIAM C. KENNEY of 367 nm. , 1971b). 3. Amino Acid Sequence at the Flavin Site The amino acid sequence of the flavin pentapeptide from a trypticchymotryptic digest of monoamine oxidase was determined using the dansyl-Edman technique (Gray, 1967). , 1971b) is: Ser-GIy-Gly-Cys(FAD)-Tyr 4.

This conclusion rests on the observation that a t the terminal stage of purification of the enzyme from pig brain the FAD can be dissociated by the acidammonium sulfate procedure and the resulting apoenzyme reactivated by adding FAD (Tipton, 1968a). While, a t first glance, this is a convincing demonstration that the FAD is indeed noncovalently bound in the pig brain mitochondrial enzyme, the situation is complicated by the report in the same paper that at earlier stages of purification the same procedure fails to extract the flavin.

24, 321. Kenney, W. , Walker, W. , Kearney, E. , and Singer, T. P. (1970). Biochem. Biophys. Res. Commun. 41, 488. Kenney, W. , Walker, W. , Kearney, E. , Singer, T. , Cronin, J . , and Hendriks, R. (1972a). 2. Nntiirforsch. B 27, 1069. Kenney, W. , Walker, W. , and Singer, T. P. (1972b). J. Biol. Chem. 247, 4510. Kenney, W. , and Singer, T. P. (1973). Biochem. Biophys. Res. Commun. 52, 434. 44 THOMAS P. SINGER AND WILLIAM C. KENNEY Kenney, W. , Edmondson, D. , and Singer, T. P. (1974a). Biochem.

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