2014-2015 Basic and Clinical Science Course (BCSC): Section by American Academy of Ophthalmology, Robert H. Rosa Jr. MD

By American Academy of Ophthalmology, Robert H. Rosa Jr. MD

Part four provides fabrics in elements: half I, Ophthalmic Pathology; and half II, Intraocular Tumors: scientific features. half I makes use of a hierarchy that strikes from basic to precise to assist derive a differential analysis for a particular tissue. half II is a compilation of chosen medical points of significance to the final ophthalmologist. Following half II are the yankee Joint Committee on melanoma 2010 staging kinds for ocular and adnexal tumors.

Upon of entirety of part four, readers will be capable to:

Describe a based method of knowing significant ocular stipulations in line with a hierarchical framework of topography, disorder method, normal analysis and differential diagnosis
Summarize the stairs in dealing with ocular specimens for pathologic learn, together with acquiring, dissecting, processing, and marking tissues
Identify these ophthalmic lesions that point out systemic illness and are possibly lifestyles threatening

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Additional resources for 2014-2015 Basic and Clinical Science Course (BCSC): Section 4: Ophthalmic Pathology and Intraocular Tumors

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36 • Ophthalmic Patho}ogy and lntraocular Tumors . These antibodies vary in their specificity and sensitivity. com). Automated equipment and antigen retrieval techniques are currently used to increase sensitivity and decrease turnaround time. Flow Cytometry, Molecular Pathology, and Diagnostic Electron Microscopy Flow Cytometry Flow cytometry is used to analyze the physical and chemical properties of particles or cells moving in single file in a fluid stream (Fig 4-3, a). An example of flow cytometry is immunophenotyping ofleukocytes.

Eye. 2001;1S(Pt 2): 143-147. Frozen Section Permanent sections (tissue that is processed after fixation through alcohols and xylenes, embedded in paraffin, and sectioned) are always preferred in ophthalmic pathology because of the inherent small size of samples. If the lesion is too small, it could be lost during frozen sectioning. A frozen section (tissue that is snap-frozen and immediately sectioned in a cryostat) is indicated when the results of the study will affect management of the patient in the operating room.

Table 4-2 Summary of Molecular Techniques Used in Diagnostic Pathology Technique Method Advantages Disadvantages SNP oligonucleotide microarray analysis (SOMA) Type of DNA microarray used to detect single nucleotide polymorphisms (SNPs), the most frequent type of variation in the genome, within a population. Uses array that contains immobilized nucleic acid sequences and 1 or more labeled allele-specific oligonucleotide (ASO) probes Amplification of multiple targets using only a single primer pair within a single PCR mixture to produce amplicons of varying sizes that are specific to different DNA sequences Chromosome regionspecific, fluorescently labeled DNA probes (cloned pieces of genomic DNA) able to detect their complementary DNA sequences Amplification of a single strand of DNA (nucleic acid) based on thermal cycles of repeated heating and co oling of the reaction for DNA melting and enzymatic replication of the DNA.

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